ABSTRACT
<p><b>OBJECTIVE</b>To distinguish female and male plants of Gynostemma pentaphyllum quickly and accurately in the stage of seedling and to understand the relation between sex differentiation and endogenous plant hormone.</p><p><b>METHOD</b>Stained methods of BTB and enzyme linked immuno-sorbent Assay were used.</p><p><b>RESULT</b>10 Hours after being dyed, the liquid extract of female plants turned into yellow, while extract of male ones turned green. There were obvious differences on iPAs and GA(1 + 3) contents but no differences on IAA and ABA contents between male and female plants during the stages of seedling and vegetative growth.</p><p><b>CONCLUSION</b>BTB can be used for distinguishing female and male plants and there were a certain relation between sex and endogenous plant hormones.</p>